Vie CONG. INTERN. REPROD. ANIM. INSEM. ARTIF, FARIS, 1968, VOL. I

Text - Fig. 1. The Effect of Glycerol on Motilityoand Cleping

of Fowl Spermatozoa kept at 37 and 3 C.

INCURATION FOR

5 min 10 min 30 min
372 3" iegg® 2 37° 5
Diluent alone 1o, 6 6 55 5 WSS
With 5% glycerol 5 6 0 6 0 6
W05 i S 6 0 Tl 0 6
" 15% " 3 6 0 6 0 4.5
" 30% " 0 3 0 3 0 3

No movement: O; Few moving and extensive clumping: 3, Vigorous
motility and few clumps: 6, Vigorous motility and no clumping: 9.

Our observations on the damage done to cells by glycerol emphas-
izes the undesirability of using motility studies as the sole criter-
ion of assessing freezing and thawing procedures for storing sperma-
tozoa, especially if motility is observed in glycerolized media at
37%. However, useful information about the efficacy of freezing
and thawing procedures can be obtained if the thawed, glycerolized
semen is kept at 2 to 300. Under these conditions good motility has
been maintained for up to 6h and this test has served as a reliable
guide as to the fertility results that can be expected with frozen
semen, after thawing and removal of glycerol, in experiments to find
methods of storing fowl semen at ultra-low temperatures.

17 SHAFFNER. C.S. 1964. Proc. 5th Int. Congr. Anim. Reprod
and A,I. Vol. 4: 426429,

2.  TANAKA, K., TOMITA, T. and OKAVOTO, S. 1966. Jap. J.
Zootec. Sci., 37: 134-138.

3. WATANABE, M. 1967. J. Fac. Fish. Anim. Husb. Hiroshima
Univie, 7 19-235.

4, Van den BERG, L. and ROSE, D. 1959. Arch. Biochem. Biophys
81: 319-329.

*9

S, Van den BERG,L. and ROSE
84: 305-315.

, D. 1959. Arch. Biochem. Biophys..

1635