TABLE 1: The agtivity of ram spermatozoa after incubation
at 37 C, chilling to 5°C or deep-freezing.

7 Motile spermatozoa

Diluent

containing 6 hro 6 dags After

185 mM of: at- 3736 at 5 deep-freezing
GLUCOSE 48 9 44
FRUCTOSE 48 30 21
ARABINOSE 38 45 21
LACTOSE 30 20 45

TABLE 2: The effects of altering the concentration of sugar
in diluents for deep-freezing ram spermatozoa

7% Motile spermatozoa

mM Sugar in diluent

185 216 247
Relative
tonicity: 0 i e
GLUCOSE 44 41 44
FRUCTOSE 30 29 Sk
ARABINOSE 28 23 29
LACTOSE 45 41 29

TABLE 3: The fertility of diluted and chilled ram semen.

Semen treatment Number of Ewes % Lambing
Inseminated
Neat semen (control) 270 67

Semen diluted in:

Glucose synthetic and
used immediately 274 58

Arabinose sgnthetic and
stored at 5 for 24 hours 283 26

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