Vie CONG. INTERN. REPROD. ANIM. INSEM. ARTIF, PARIS, 1968, VOL. I

Table I - Diluent and procedure for extending
Equine Semen

VOLUME, COMPLETE ML. 100
EGG YOLK ML. 20
LACTOSE (BACTO) GM. 320
LEVULUSE GM. 2o D
SORBITOL GM. 0.08
INOSITOL GM. 0.08
ANTIBIOTICS

Penicillin 50,000 units

Streptomycin 50,000 M 9.
GLYCERINE RANGE E 506505, 3 (1)
pH - 7.0 (2)

(1) Complete with semen
4X Dilution - Use 7.8%
5 to 6 X - Use 7.0%
7 to 10 X - Use 6.5%

INTERPRETATION:

The final mixture of processed semen should
contain glycerine within a range of 5.6 to 5.8
percent. When one part of semen is diluted to
5 X, it would require 4 parts of a diluent con-
taining 7% glycerine plus the one part of semen
to equal a final mixture of 5.6% glycerine.

(2) Adjust with 5% NaHCO3 to 6.9 pH.

After two days under refrigeration (5°C.)
thisSwitlF = driE et ot S i pDH

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