Vie CONG. INTERN. REPROD. ANIM. INSEM. ARTIF., PARIS, 1968, VOL. I THE EFFECTS ON EQUINE REPRODUCTIVE FUNCTION OF UTERINE INFUSION OF SALINE G.H. ARTHUR Department of Surgery and Obstetrics, Royal Veterinary College, London, England. In veterinary practice non-pregnant mares which do not show heat may be cases of true anoestrus with cyclical inactivity, or they may be cycling mares which do not manifest heat - so-called suboestrus, or "silent heat", cases. When a veterinary surgeon is asked to examine and treat a mare which does not show heat it is im- possible for him to state on the basis of a single gynaecological examination of the ovaries and cervix whether the mare is anoestrous or dioestrous. Only the suboestrous mares that happen to be in silent heat at the time of the examination can be identified; all the remainder tend to be put into the same category of "anoestrus!'. The present in- vestigation was designed to clarify the responses at different times of the year of both anoestrous and dioestrous mares to uterine infusion of saline and, in particular, to find out whether ovulation accompanied the heats which might be induced in both these phases of reproduction. MATERIALS AND METHODS. Five of the mares used were of the Welsh Mountain breed and three were Thoroughbreds; their ages ranged from 5 to 10 years. The mares were in good bodily condition. They wintered out-of-doors when hay was fed but during Spring and Summer they were housed during the day and turned out at night. The investi- gation extended from February 1967 to May 1968. Two of the mares were treated at approximately monthly intervals throughout the experiment. The remaining 6 mares were treated during the breeding seasons of 1967 and 1968 and of these, 3 had foaled during 1967 and 3 were barren that year. The reproductive status of each mare at the time of treatment was known from previous gynaecological examinations. 1551