Vie CONG. INTERN. REPROD. ANIM. INSEM. ARTIF., PARIS, 1968, VOL. I

result indicates a promoting effect of TPD on the respiration
of spermatozoa. At the concentration of 294 ppm which was
harmful to sperm viability, oxygen consumption was low.

Table 1. Effect of addition of TPD on the 2nd or 4th day
of storage on viability of bull spermatozoa.

 

 

Day of Days of storage
addition Lot 8 o sani R 5
2nd day Controli e, 00 7o raubb gtz 5E P 5 aRlE
(n=8) TPD add. - - ¥ 80 73 62 50 36
Lktn day Controley 788 77. 75 i65aa5 i S 6 0T 52
(n=b) SRR adl) e T

Note: Sperm viability is shown by sperm motility index.
n is number of trials. +means addition of TPD.

Table 2. Effect of TPD on sperm motility after thawing.

Storage at 4°C after thawing (hrs.)
Animal Lot

 

0 12 2L
Bull Control 58 55 Ls 53
(n=6) TPD add.. 63 64 63 58
Goat Control L2 41 3L 20
(n=4) TPD add. 50 59 61 58

Table 3. Penetration of thiamine and TPD into bull

spermatozoa.
Incubation Thiamine TPD Control
time lot lot (no addition)
Just after
addition Qez: . 08 el g
0.25 hr. 0.3 Tl
50 052 2l -
2500 055 2 -
6.00 042 9 -
12.00 G2 257 -
24 .00 0:2 2.4 -

Note: Figures show thiamine concentration (ug) per 100
million of spermatozoa. Concentration of thiamine
and TPD used is 63 ppm, respectively.

1289