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The effect of citric acid on the vitality of
bovine spermatozoa

G. KICHEV, P. MARINOV, G. KALEV, Z. ZAKHARIEV,
S. ZLATAREV

T Babaile i bbiieninimSeTia “F3TBul saria

It was established that, in order to raise the hydro-
gene-ionic concentration of the yolk-glucose-citrate
diluter,from pH = 7,00 to pH = 6,40, it is necessary to
add 10" ° 2,62 Mol/ml citric acid ; and to raise it from
PH = 6,40 to pH = 6,00, it is necessary to add 10
3,175 Mol/ml citric acid.

In 66,5 % of the ejaculates the addition of citric acid
caused an activation of oxygen uptake and detention of the
reductive capacity of spermatozoa. In the remaining
35,5 % this dependance was the opposite, i,e, a depression
of oxygen uptake and activation of reduction. When larger
quantities of citric acid were used, a protraction of
the reduction period was observed in all the ejaculates.
In 42,5 % of the ejaculates, increase of survival was
observed in correlation with the concentration of citric
2,6

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