Commercially obtained a-aminocaprylic acid gives a
spot upon the chromatograms on the same place as "B".
"B" is being isolated and until it is obtained in a
well crystallized,pure form, which can be investigated
with I.R. and other means, complete certainty about
its composition cannot be obtained; result 6 point
also at the probability, that "B" is identical with
a-aminocaprylic acid. "B'" occurs mainly in the sper-
matozoa, probably bound to carbohydrates. "B" is libe-
rated by boiling spermatozoa with 2N HCl. Possibly two
isomers are formed."B" does not occur free.

RESULTS INCUBATION EXPERIMENES
l.Incubation of washed spermatozoa with seminal
plasma did not result in a considerable change of thae
keto acid composition,whereas after incupation of
homogenized spermatozoa witn seminal plasma a large
increase of the amounts of keto acids both quanti-
tatively as qualitatively could be observed.
2.After incubation of nomogenized "normal'spermatozoa
with "normal" seminal plasma,a-ketoglutaric acid,
a-keto-leu and a~keto-B were present; after incuba-
tion of homogenized "bad" spermatozoa with "bad"
seminal plasma also rather large amounts of phenyl-
pyruvic acid occurred.
3,After incubation of homogenized spermatozoa with
water,a-keto-B and n-keto-leu were present and ax-keto-
glutaric acid and pyruvic acid only in very small
amounts. Phenylpyruvic acid was absent.
4.Incubation of homogenized spermatozoa and  phe with
water or with seminal plasma resulted in all cases
in formation of phenylpyruvic acid.
5.Incubation of seminal plasma with phe did not
result in formation of phenylpyruvic acid.
6.Tyrosine,leu and a-aminocaprylic acid were converted
in respectively p-hydroxyphenylpyruvic acid,x-keto-
leu and in a-keto-B after incubation of homogenized
spermatozoa with water,but valine and alanine
were not converted.
7T.After homogenization with water only the sperma-
tozoa residues could produce transamination; the
watery solution could not.

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