Vle CONG. INTERN. REPROD. ANIM. INSEM. ARTIF., PARIS, 1968, VOL. li

during this experiment,

The question remains to what extent the observations made on the
fertilization process are reliable and to what extent we have to do
with artifacts.

The C.S.'s influence on the acrosome is instantaneous. It could not be
decided whether the spermatozoa's dying off comes first and is follow-
ed by vesiculation, or whether vesiculation results in the spermato-
zoa's dying off.

Up till now it has not been clear in which way the C.S. is causing this
effect. We assumed it might be a result of osmotical difference.
Experiments to settle this question produce however negative results.
As far as I know no other phenomenon is reacting in this way towards
a sudden drop in temperature in cell-biology.

Literature

1.C.R. Austin J. Reprod. Fertil. (1963) 6 p. 313-314
2.G.H.B. Teunisse Tijdschrift Diergeneesk. (1946) 71 p. 292-297
3.J.L. Hancock J. Exp. Biol. (1953) 30 p. 50-58

4,A., Bane Proc. IVth Int. Congress Anim. Reprod.
The Haque (1961) p. 810

5.R. Hadek J. Ultrastruct. Res. (1963) 8 p. 161

6.P.N. Srivastava c.s. J. Reprod. Fert. (1965) 10 p. 61-67

7.J.M. Bedford J. Reprod. Fert. (1963) 6 p. 245-255

8.Don W. Fawcett c.s. Zeitschrift f. Zellforsch. (1963) 60

p. 276-292
J.M. Bedford J. Reprod. Fert. (1963) 5 p. 169-177
10.J. Boender Jaarverslag Centrale Comm. K.I. 1963 p. 30-38
11.J.L. Hancock J. Royal Microsc. Soc. 76 (1957) p. 84-97
J

12,J.” Boender World Review of Animal Prod. Spec. Issue II

1966 p. 29-44

13.J.L. Hancock Advances in Reprod. Physiol. vol. I (1966) Acad.
Press Ed. by A. McLaren p. 125-154

14, L. Nicander and A. Bane Z. Zellforsch. Mikrosk. Anat. (1962)
57 p. 390-398

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