VIe CONG. INTERN. REPROD. ANIM. INSEM. ARTIF., PARIS, 1968, VOL. Il

(1) C.S. SHAFFNER, E.W. HENDERSON and C.G. CARD, 1941. Poult. Seci.,
20:259- -265.

(2) C.S. SHAFFNER, 1942. Science, 96:337.

(3) c. POLGE, A.U. SMITH and A.S. PARKES, 1949. Nature, @:666.

(4) A.U. SMITH and C. POLGE, 1950. 1ibid., 166 :668.

(5) A.S. PARKES, 195C. Proc. Soc. Stud. Fertil. (Camb), 2:12-15.

(6) C. POLGE, 1951. Nature, 167:949.

(7) T.E. ALLEN and L.W. BOBR, 1955 Poult.=Sele; l 1167-1169

(8) T.E. ALLEN, 1958. Proc. Aust. Soc. Anim. Prod., 2: :118-119.

(9) C.E. CLARK and C.S. SHAFFNER, 1960. Poult. Sci., :1213-1220.

(10)J.E. BROWN and C.G. HARRIS, 1963. ibid., _@:3}7-} 0

(11)C.S. SSHAFFNER, 1964. Proc. 5th. Inter. Cong. Anim. Reprod. A.I.
4:426-429,

(12)K.” TANAKA, T. TOMITA and S. OKAMOTO, 1966. Jap. J. Zootech.
Sei., 37:134-138.

Table 1. The composition, pH and depression of freezing point of diluents
used for this experiments

 

 

 

Diluents pH 1 Depression of freezing point (—°C)
A* 6.2 ' 0.58
Bx* 6.3 0.52
Cres 6.3 } 1.11
Dkx 6.5 1.08
Yamane’s solution 7.0 1.08
Locke-Ringer solution 71 0.61

 

Composition :
*: 59¢, C4H,,0; solution 85 plus fresh egg yolk 15

s ” 95 ” 5
**%: 99, CoH,,0, solution 85 plus fresh egg yolk 15
RS ” 95 ”

Yamane’s solution: One volume 6.5% Na,C,H;0;. 2H,O solution plus one volume fresh
egg yolk plus two volume 9.0% CgH,;0; solution

Locke-Ringer solution: NaCl 9.0g, KCl 0.42g, CaCl, 0.24g, dextose 0.5g, MgCl; 0.2g and
NaHCO; 0.5g per distilled water 1,000 ml.

Diagram 1. Temperature change in freezing and thawing of the chicken semen
A) Four freezing methods of the chicken semen
1) 37°C (diluted with diluent, addition of glycerol) ---~> 20°C (glycerol equilibration) —
-79°C
2) 37°C (diluted with diluent) ----> 20°C (glycerol addition and equilibration) — -79°C
3) 37°C (diluted with diluent) -----» 20°C (glycerol addition and equilibration) --->» 0°C —

 

 

 

 

 

 

-79°C
4) 37°C (diluted with diluent) ---~> 5°C (glycerol addition and equilibration) ---» 0°C —

- -79°C

B) Four thawing methods of the chicken semen

a) -79°C - 37°C
b) -79°C - 5°C (keeping gently for 5mins.) ——M M, 37°C
c) -79°C - — - 20°C
d) -79°C — 5°C (keeping gently for 5 mins.) 20°C

Remarks:

...... > shows the falling of temperature 1°C/minute
> shows the quick rising and falling of temperature

 

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