Vle CONG. INTERN. REPROD. ANIM. INSEM. ARTIF., PARIS, 1968, VOL. Il

APPLICATION OF EOSIN FOR DIFFERENTIAL STAINING OF BUFFALO
SPERMATOZOCA
Part II. Stain concentration and sperm differentiation in buffa-
loes.
NOUR EL-DIN A. ALY, YOUNES, A.A., ABBOUD, M.Y., OMAR, M.A.
Animal Science Research Institute, U.A.R., Dokki.

The concentration of the stain, used for differential staining
of buffalo spermatozoa, in the process of semen evaluation, has a
critical 4importance, in selecting ejaculates for artificial insemi-
nation. The present investigation was carried out to determine the
optimum concentration of eosin yellowish which proved to be more
efficient in the process of buffalo semen evaluation, for differen-
tial staining of the cells. A concentration of 2% eosin yellowish,
in Part I was found to be suitable. The difference between concen-
trations was more or less large (1%). A more suitable concentration
of the stain may exist nearby and not exactly 2%. Accordingly another
investigation was necessary to perform. This work also informs about
the correlation between percentage of live sperm and stain concentra-

tion.

Experimental.ll concentrations of eosin yellowish were checked
starting with 1.5% and till 2.5%. The difference between concentra-
tions was O.l%. 77 smears of buffalo semen collected from 4 buffalo
bulls, were examined microscopically after motility determination

and staining with the different concentrations of eosin yellowish.

Results. 1. Average motility ratings was 71.43-2.00%.

2.The highest percentage of live sperm was 72.2410.88%, obtained by
using 1.7% eosin yellowish.

3. 1.6% eosin yellowish led also to a high percentage of live sperm
(70.0020.83%) .

4.The lowest percentage of live sperm was 29.75:0.96%, obtained by

using 2.5% solution.

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