3.The lowest percentage of live sperm was 54.36:0.21%, obtained by
using congo red - eosin.
4.The use of methyl blue-eosin led to intermediate results. The
percentage of live spern was 57,9120, 21%.
S5.Nigrosin imparted a dark blackish background to the smear, methyl
blue imparted a bluish one and congo red a reddish background.
6.The use of the 3 compound stains led to different correlations
between motility and percentage of live sperm. In case of eosin-
nigrosin there was a significant positive correlation, r=+0.968,
with methyl blue-sosin r=+0.089 and with congo red-eosin r=-0.029,
Among the above checked compound stains, eosin-nigrosin was
more suitable as it led to the highest percentage of live sperm,
more coinciding with motility. The use of eosin-nigrosin alsoc led
to a high positive correlation between motility and percentage of
live sperm. Moreover by applying nigrosin as a background stain,
a better field for sperm differentiation was obtained by providing
contrast to the sperm. Nigrosin imparted a dark blackish background,
facilitating the differential count of buffalo spermatozoa. Sone
morphologic studies could be nerformed on buffalo spermatozoa with

a stained background.

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