supernatant removed and the sperm rediluted to approximate the volume
of the unmixed pooled sample collected immediately after thawing.
Re-extended sperm in CME was poured into 2 ml. plastic vials and
stored in the dark at room temperatures. Lyophylization of coconut
milk (water) was done as described by D. GROVE (3).

RESULTS.,

Table 1. Summary of Conception Results in Countries
Utilizing the CME Technique

Years CME
has been

No. Ist

Inseminations

207715 13, 405

Country

45,000 30,150 67 (based on 60-90
day non-return)
8,900 5,073 57(based on Preg-
Mysore, 61-bosed on Preg-
India nancy examinations
Westx x 3,869 2,790 72(based on 60-90
Germany day non-return)

® % conception is average for sperm which were 1-5 days old at the
time of insemination

% % Inseminations made with reconstituted lyophylized coconut milk
(water) which had been stored 1-2 yrs. prior to reconstitution.

 

Table 1 indicates that in those countries which have adopted CME
for routine use in their A.l. centers during the past five years the rate
of conception has, without exception, been maintained at a satisfactory
level. The higher rate of non-return reported for West Germany may be
attributed to the better nutritional level of the animals involved and
better control of diseases effecting reproductive performance. Dr.

G rove who conducted the experiment pointed out that all of the experi-
m ents were split sample experiments, and that the room temperature

120