D etermination of pregnancy was made by rectal palpation three months
after insemination with CME.

RESULTS.

Table 1.  Motility of Murrah Buffalo Sperm kept at Room
Temperatures in CME for seven days.

e e b btk 20 Tind| @Hobivs 1o A7 Zihess ol 1120 dkescs 11680hrs,
Bulls efdculates) s A oBiil nA B TA Bl Al B Jl A B

708 4.18(67.5 3.96| 60.4 3.74( 57.8 3.60| 56.0 3.35

A . Percent motile sperm
B. Quality of motility based on a scale of 0 (non-motile) to 5
(excellent progressive motility).
N ote: Samples which revealed frank contamination are not included
in table.

 
  
  

  
  

  

  

Table 2.  Pregnancy Results for Murrah Buffalo Inseminated
with 1 to 5 day old semen kept in CME at Room

Temperatures.
No. of Ist No. of Buffalo
S, % Pregnant
Inseminations Pregnant
144 89 62

 

The evaluation of the motility of buffalo sperm is given in Table 1.
Quite often one or more ejacultes from the same bull had to be dis-
carded because of a high percentage of dead sperm. This is commonly
observed in buffalo during the non-breeding season which lasts from
January to August. It can be seen that ejaculates starting out with high
numbers of motile sperm could be conserved for seven days with a re-
latively small reduction in the number of motile cells and in the quality
of their motion. Table 2 presents the pregnancy data for a small number
of Murrah she-buffaloes which were rectally palpated three months after
they had been inseminated with 1 - 5 day old semen kept in CME at
room temperatures. Out of 144 first inseminations 89 or 62% were found
pregnant, These figures must be regarded as tentative until numerically
larger and more significant figures become available.

These results are encouraging inasmuch as they indicate that buffalo
sperm motility and fertilizing capacity can be maintained for longer per-
iods in CME than in milk and egg yolk extenders. It is also evident that
the advantages of artificial insemination can be achieved more economi-

mé