The post-thaw motility of the spermatozoa was very low when
egg-yolk polyol solution were used as freezing extenders without
any basic solutes.

Fig. 2 indicates that the post-thaw motility in a trial
testing xylitol effects in isotonic basic solutions, such as
citrate, tris buffer and glucose media containing 20% yolk.

Although the best survival level in each medium was
different, the best motility in the post<~thaw semen was about
the same in three basic solutions and was improved markedly
compared to those in egg-yolk xylitol solution without basic
solutes.

REFERENCES

(1) A. U. SMITH and C. POLGE, 1950, Nature, 166,668

(2) C. W. EMMENS and A. W. BLACKSHAW, 1950, Aust. Vet, J. 26,226
(3) H. NAGASE, 1966, Japan Agric, Res, Quart, 1, No. 2, 1

(4) TI. S, DAVIS, R, W, BRATTON and R, H, FOOTE, 1963, J, Dairy
Sci. 46,324

(5) C. POLGE and M. A, SOLTYS, 1960, Recent. Res. Freez. and
Dry. ed. parks and Smith, 87, Blackwell Sci. Publ.

(6) H. NAGASE, 1966, Jap. J, Anim, Reprod., 11 (supplement),
2, (In Japanese)

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