CONCLUSION, Based on the conditions and results of this trial, it
would appear that it would be advantageous to freeze semen packaged
in plastic straws by a method employing a liquid medium for heat
transfer, This observation is similar to effects observed
previously for bovine semen packaged in glass ampoules (&),

TABLE I
ANALYSIS OF VARTANCE FOR PER CENT OF LIVE

SPERMATOZOA IN SAMPLES PACKAGED IN STRAWS

Source daf aeMoSet -
Freezing Method (A) 1 8431 ,87%
. Extender (B) 1 2235
Time of evaluationl (C) 1 889,90
AxB 1 565,76
AxC ! 25,63
BxC g 3282
AxBxC 1 8,10
Residual 544 597e85

1 Before and after 5 hours incubation,

* P e=404054
REFERENCES,

1, Macpherson, J, W, (1960), Sterile Milk As A Semen Diluent,
Gansr Vetlhudoures Vol d simes i1 2s

2, Rajamannan, A, H, J., Graham, F,, and Smith, F, (1964). The
Effect of a New Extender Incorporating Dried Egg Yolk On The
Fertility of Bovine Spermatozoa, V Congress Int, An, Reprod,
and Art, Insemination, Trento,

3, Hackett, A, J,, and Macpherson, J, W, (1964), A Method For
Differential Staining Of Bovine Spermatozoa After Extension In
Sterile Milk, 'Can, Vet, Jour, Vol, 6, noe 5.

4, Macpherson, J, W, (1962), Some Observations of Procedures For
Freezing And Handling Bovine Semen, XXV Ano, Instito
Spermentale Italiano,

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