VIe CONG. INTERN. REPROD. ANIM. INSEM. ARTIF., PARIS, 1968, VOL. I The area has achieved a 90/120 day non-return rate over 18,000 inseminations of 68.1%. Pelleted semen and lst day liquid semen was used at the same time in our mainland Sub-Centres from September 1965 to July 1967. Some 2nd day liquid semen was used early in the period but was discontinued owing to the lower conception rate obtained. TABLE III A comparison of the conception rate obtained with lst and 2nd day liquid semen with that of pelleted semen (9Q/12O day ). _ Pellet 1st day liquid |2nd day liquid 65.1 67.4 63.1 ] | Inseminations The pellet conception rate was significantly lower than that of first day liquid semen but did not differ significantly from 2nd day rate. It has been reported by Isbister (3) that the decline between 30/60 day and 90/120 day non return rates is significantly greater (1.9%) for semen frozen in ampoules. The comparable figures for fresh and pelleted semen shows no significant difference. TABLE IV A comparison of 30/60 day and 90/120 day non return rate for liquid and pelleted semen (December 1965 to November 1966 ). Number of 1lst| 30/60 day 90/120 day Inseminations Non return | Non return Difference % Non=return Pellet 25,663 Liquid 37,071 P> 0.3 Out of 1458 ejaculates frozen only 17 were unsuitable for use It was found that the average percentage of live spermatozoa before freezing was 72% and the average live sperm lost during the freezing process was 18%. The results showed that the non return rate was not affected by the numbers of live spermatozoa per pellet within a range of 10 - 35 million. Discussion The freezing of bovine semen in pelleted form provides an acceptable alternative to liquid semen. Although the pellets did not quite obtain the fertility level of liquid semen used on the day of collection, the wastage of semen was reduced and maximum use could be made of outstanding sires. There appears to be no advantage gained from the addition of antibiotics to the pelleted semen. 1023